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Immunohistochemical diagnosis of typhus rickettsioses using an anti-lipopolysaccharide monoclonal antibody.
Immunohistochemical diagnosis of typhus rickettsioses using an anti-lipopolysaccharide monoclonal antibody.
- Source :
-
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc [Mod Pathol] 1997 Oct; Vol. 10 (10), pp. 1038-42. - Publication Year :
- 1997
-
Abstract
- A monoclonal antibody directed against an epitope on the lipopolysaccharide of typhus-group rickettsiae was developed for the purpose of detecting this heat-stable, proteinase-resistant antigen in formalin-fixed, paraffin-embedded tissues. Rickettsia prowazekii organisms were identified in endothelium and macrophages in sections of the brains of three Egyptian men who died of epidemic louse-borne typhus in Cairo during World War II and in the brain from a recent case of typhus fever acquired in Burundi. R. typhi organisms were identified in endothelial cells from a fatal case of murine typhus and in experimentally infected mice. This approach is applicable not only to the study of archival tissues and experimental animal models but also could be used to establish a timely diagnosis of typhus-group rickettsiosis by immunohistochemical examination of cutaneous biopsies of rash lesions during the acute stage of illness.
- Subjects :
- Adolescent
Adult
Aged
Animals
Brain blood supply
Brain microbiology
Brain pathology
Endothelium, Vascular microbiology
Endothelium, Vascular pathology
Fatal Outcome
Female
Formaldehyde
Guinea Pigs
Humans
Immunohistochemistry methods
Male
Mice
Paraffin Embedding
Rickettsia prowazekii isolation & purification
Rickettsia typhi isolation & purification
Antibodies, Monoclonal
Lipopolysaccharides immunology
Rickettsia prowazekii immunology
Rickettsia typhi immunology
Typhus, Endemic Flea-Borne diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0893-3952
- Volume :
- 10
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 9346184