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Regulated expression of 5'-deleted mouse GLUT4 minigenes in transgenic mice: effects of exercise training and high-fat diet.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Oct 20; Vol. 239 (2), pp. 503-9. - Publication Year :
- 1997
-
Abstract
- Fourteen kb murine GLUT4 minigene (= -7395 GLUT4) contains DNA sequence that confers tissue specific, exercise-induced up-regulation of the GLUT4 gene in skeletal muscle and high-fat diet induced-down-regulation in white adipose tissue. To identify the DNA sequences required for regulated expression, we generated GLUT4 minigene transgenic mice harboring 3237, 2000, 1000, and 442 bp of 5'-flanking region, all exons and introns, and 1 kb of 3'-flanking sequence of the mouse GLUT4 gene. The -3237-, -2000-, and -1000-GLUT4 constructs were expressed in a tissue-specific manner identical to the endogenous GLUT4. Exercise-induced up-regulation and high-fat diet-induced down-regulation of these constructs also paralleled those of the endogenous GLUT4 gene. In contrast, the -442 GLUT4 construct was expressed substantially in skeletal muscle (gastrocnemius and quadriceps) and heart, but was only expressed very weakly in white adipose tissue and was not expressed in brown adipose tissue. Furthermore, this -442 GLUT4 construct failed to respond to exercise or a high-fat diet in either muscle or adipose tissue. These results indicate that brown and white adipocyte-specific enhancer(s) and exercise- and high-fat diet-responsive elements are located between bases -1000 and -442 of the murine GLUT4 5'-flanking region.<br /> (Copyright 1997 Academic Press.)
- Subjects :
- Animals
Dietary Carbohydrates administration & dosage
Eating physiology
Female
Glucose Transporter Type 4
Male
Mice
Mice, Transgenic
Monosaccharide Transport Proteins biosynthesis
Dietary Fats administration & dosage
Gene Expression Regulation physiology
Monosaccharide Transport Proteins genetics
Muscle Proteins
Physical Conditioning, Animal
Sequence Deletion physiology
Transgenes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 239
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9344860
- Full Text :
- https://doi.org/10.1006/bbrc.1997.7516