Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages.

Authors :: Denoel PA
Crawford RM
Zygmunt MS
Tibor A
Weynants VE
Godfroid F
Hoover DL
Letesson JJ
Source :: Infection and immunity [Infect Immun] 1997 Oct; Vol. 65 (10), pp. 4337-40.
Publication Year :: 1997
Abstract: A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.

Subjects :: Brucella melitensis genetics
Brucella melitensis growth & development
Gene Deletion
Genetic Complementation Test
Humans
Macrophages microbiology
Monocytes microbiology
Mutation
Oxidative Stress
Species Specificity
Bacterial Proteins
Brucella melitensis pathogenicity
Cytochrome b Group genetics
Ferritins genetics
Macrophages immunology
Monocytes immunology

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