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Mineralized nodule formation by human dental papilla cells in culture.
- Source :
-
European journal of oral sciences [Eur J Oral Sci] 1997 Aug; Vol. 105 (4), pp. 318-24. - Publication Year :
- 1997
-
Abstract
- Human dental papilla cells were enzymatically separated from deciduous tooth germs of an 8-month-old embryo legally aborted. The second passage cells were cultured up to 35 days in 3 groups. The beta-GP group was cultured in the Dulbecco MEM containing ascorbic acid and beta-glycerophosphate supplemented with 15% fetal bovine serum. The Dex group was in the same medium, in addition containing dexamethasone. The control group contained none of the 3 chemicals. Mineralized nodules were formed after 15 days in the beta-GP and Dex groups. Only in the presence of ascorbic acid and organic phosphate did they mineralize. The addition of dexamethasone caused a significant increase in the number of nodules. By electron microscopy, the nodules contained needle-shaped crystals associated with a network of collagen fibrils. Calcium and phosphorus were detected by energy-dispersive X-ray microanalysis in the nodules. Furthermore, the crystalline material exhibited a pattern consistent with hydroxyapatite and dentin when examined by X-ray diffractometry. Cells showed high levels of alkaline phosphatase activity, which was increased 2-3 times in the presence of the 3 chemicals. These results indicated that human dental papilla cells have the ability to form dentin in culture. The formation of mineralized nodules by human dental papilla in vitro provides a useful model for studying the morphogenesis and differentiation of dental papilla ectomesenchyme.
- Subjects :
- Alkaline Phosphatase analysis
Alkaline Phosphatase drug effects
Ascorbic Acid pharmacology
Calcium analysis
Cell Differentiation
Cells, Cultured
Collagen ultrastructure
Crystallography
Culture Media
Dental Papilla drug effects
Dental Papilla embryology
Dental Papilla ultrastructure
Dentin chemistry
Dentin ultrastructure
Dentinogenesis
Dexamethasone pharmacology
Durapatite analysis
Ectoderm physiology
Electron Probe Microanalysis
Glucocorticoids pharmacology
Glycerophosphates pharmacology
Humans
Male
Mesoderm physiology
Microscopy, Electron
Morphogenesis
Phosphorus analysis
Tooth Calcification drug effects
Tooth Germ embryology
Tooth, Deciduous embryology
X-Ray Diffraction
Dental Papilla physiology
Tooth Calcification physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0909-8836
- Volume :
- 105
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of oral sciences
- Publication Type :
- Academic Journal
- Accession number :
- 9298363
- Full Text :
- https://doi.org/10.1111/j.1600-0722.1997.tb00247.x