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Monoclonal antibody against species-specific epitope of Pseudomonas aeruginosa Hsp60 protein cross-reacts with Pseudomonas stutzeri and other Pseudomonas species.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 1997 Sep 01; Vol. 154 (1), pp. 131-7. - Publication Year :
- 1997
-
Abstract
- In a previous study, we determined the epitope of the Pseudomonas aeruginosa Hsp60 heat shock protein which is recognized by the specific monoclonal antibody (MAb) 2528. Subsequent investigations revealed a weak cross-reactivity of MAb 2528 with P. stutzeri, P. alcaligenes, P. mendocina and P. pseudoalcaligenes. To elucidate the molecular structure for these cross-reactions, we cloned the P. stutzeri hsp60 gene in Escherichia coli and determined the nucleotide sequence of the gene. In addition, the hsp60 gene of further Pseudomonas species was amplified and sequenced and amino acid substitutions within the epitope recognized by MAb 2528 were determined. The decapeptide QADIEARVLQ is unique to the P. aeruginosa Hsp60 protein, and cross-reaction of MAb 2528 reflects the phylogenetic relationship of Pseudomonas species as P. aeruginosa and all four cross-reacting species constitute a DNA homology group within the rRNA group I of the family Pseudomonadaceae, which belong to the gamma-subclass of the Proteobacteria.
- Subjects :
- Amino Acid Sequence
Antibody Specificity
Bacterial Proteins analysis
Bacterial Proteins genetics
Bacterial Proteins immunology
Blotting, Western
Chaperonin 60 analysis
Chaperonin 60 genetics
Cloning, Molecular
Cross Reactions
DNA, Bacterial analysis
Epitopes analysis
Molecular Sequence Data
Pseudomonas aeruginosa chemistry
Pseudomonas aeruginosa genetics
Sequence Analysis, DNA
Species Specificity
Antibodies, Monoclonal
Chaperonin 60 immunology
Epitopes immunology
Pseudomonas aeruginosa immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 154
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 9297831
- Full Text :
- https://doi.org/10.1111/j.1574-6968.1997.tb12634.x