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Comparison of pediatric and adult IgE antibody binding to fish proteins.
- Source :
-
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology [Ann Allergy Asthma Immunol] 1997 Aug; Vol. 79 (2), pp. 131-7. - Publication Year :
- 1997
-
Abstract
- Background: Allergic reactions to fish are a common cause of food allergy.<br />Objective: We compared the binding of pediatric and adult fish-allergic patient IgE antibodies to fish proteins.<br />Methods: Clinical histories of fish allergy were confirmed by prick skin tests, RAST and if possible, with blinded oral food challenges. The patients included five children with severe allergic reactions to catfish (4/5), cod (1/5), and tuna (1/5) and five adults with severe allergic reactions to catfish (5/5), cod (2/5), snapper (3/5), and tuna (2/5). Extracted proteins from catfish, cod, snapper, and tuna were separated with SDS-PAGE. IgE immunoblots and immunoblot inhibition studies were performed using serum sample from these patients.<br />Results: Multiple fish proteins ranging from 12 to 45 kD from the four fish extracts were identified by SDS-PAGE. A major protein (12.5 kD) was present in all fish extracts except for raw tuna. Immunoblots using individual pediatric and adult serum samples revealed that the major IgE binding was to the 12.5-kD protein from catfish, cod, and snapper. The immunoblot with tuna using serum from a pediatric patient with isolated tuna anaphylaxis revealed an IgE binding protein band at 40 kD. Preincubation of serum samples from two separate fish-allergic patients with 1 mg of cod fish extract completely inhibited IgE binding to the 12.5-kD fish protein in subsequent immunoblots.<br />Conclusions: Pediatric and adult fish-allergic patients have similar in vitro IgE binding to a 12.5-kD protein from fish extracts. This protein is immunochemically similar to Gad c I, the major allergen in cod.
- Subjects :
- Adult
Animals
Binding Sites, Antibody
Catfishes
Child
Child, Preschool
Female
Fishes
Food Hypersensitivity blood
Food Hypersensitivity etiology
Humans
Immunoblotting
Immunoglobulin E immunology
Male
Middle Aged
Proteins immunology
Radioallergosorbent Test
Skin Tests
Tissue Extracts adverse effects
Tuna
Immunoglobulin E blood
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1081-1206
- Volume :
- 79
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9291417
- Full Text :
- https://doi.org/10.1016/S1081-1206(10)63099-2