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Prostaglandin E2 is the major arachidonic acid metabolite secreted by esophageal mucosal cells in rabbits.
- Source :
-
Inflammation [Inflammation] 1997 Aug; Vol. 21 (4), pp. 419-29. - Publication Year :
- 1997
-
Abstract
- Unlike gastric mucosa, it has been considered that lipoxygenase metabolites protect the esophageal mucosa and that prostaglandins are only secreted in the presence of esophageal inflammation. The aim of this study was to determine the profile of arachidonic acid metabolites and their response to regulatory compounds in rabbit esophageal mucosal cells in culture. Eicosanoids secreted into the medium were extracted and identified by HPLC and RIA. Esophageal mucosal cells in culture metabolized arachidonic acid mainly through the cycloxygenase pathway and PGE2 was the major arachidonic acid metabolite secreted. The addition of IL-1 beta and A23187 (calcium ionophore) stimulated PGE2 synthesis. In basal conditions neither leukotrienes nor HETEs were detected. However, the addition of the NDGA induced the secretion of lipoxygenase metabolites identified as 12-15 HETEs. In conclusion, rabbit esophageal epithelial cells in culture metabolize arachidonic acid via both cycloxygenase and lipoxygenase pathways. In our system, PGE2 was the main arachidonic acid metabolite.
- Subjects :
- 6-Ketoprostaglandin F1 alpha metabolism
Animals
Arachidonic Acid antagonists & inhibitors
Calcimycin pharmacology
Cells, Cultured
Cyclooxygenase Inhibitors pharmacology
Esophagus cytology
Indomethacin pharmacology
Interleukin-1 pharmacology
Lipoxygenase Inhibitors pharmacology
Masoprocol pharmacology
Mucous Membrane cytology
Mucous Membrane metabolism
Rabbits
Arachidonic Acid metabolism
Dinoprostone metabolism
Esophagus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0360-3997
- Volume :
- 21
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 9276764
- Full Text :
- https://doi.org/10.1023/a:1027318520752