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Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. thompsoni is required for the formation of inclusion bodies.
- Source :
-
FEBS letters [FEBS Lett] 1997 Aug 04; Vol. 412 (3), pp. 587-91. - Publication Year :
- 1997
-
Abstract
- Cooperation of two crystal proteins from Bacillus thuringiensis subsp. thompsoni. strain HnC was shown to be essential for the formation of inclusion bodies. Expression of the operon containing the 34-kDa and 40-kDa protein genes from HnC in a B. thuringiensis crystal minus strain resulted in the formation of inclusion bodies identical to those from strain HnC. Interruption of one of the genes in the operon led to the lack of inclusion body and to low production of the remaining protein. Absence of inclusion body and low rate of protein production were also observed when both genes were simultaneously expressed but on different vectors. To show a cooperative effect in the formation of the inclusion body, both proteins must be produced from the same transcript.
- Subjects :
- Bacillus thuringiensis chemistry
Bacillus thuringiensis genetics
Bacillus thuringiensis Toxins
Bacterial Proteins genetics
Bacterial Proteins physiology
Endotoxins genetics
Endotoxins physiology
Gene Expression Regulation, Bacterial
Genes, Bacterial
Hemolysin Proteins
Inclusion Bodies chemistry
Inclusion Bodies genetics
Molecular Weight
Operon
Spores, Bacterial chemistry
Spores, Bacterial metabolism
Bacillus thuringiensis metabolism
Bacterial Proteins biosynthesis
Bacterial Toxins
Endotoxins biosynthesis
Inclusion Bodies metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 412
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 9276472
- Full Text :
- https://doi.org/10.1016/s0014-5793(97)00866-1