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Immunogenic, non-infectious polio subviral particles synthesized in Saccharomyces cerevisiae.
- Source :
-
The Journal of general virology [J Gen Virol] 1997 Aug; Vol. 78 ( Pt 8), pp. 1829-32. - Publication Year :
- 1997
-
Abstract
- Polioviral genes coding for P1, the precursor for the structural proteins, and 3CD, the viral protease, were cloned in a Saccharomyces cerevisiae inducible expression system. N-antigenic empty capsids could be isolated from the yeast cell extract provided that pirodavir, a capsid-binding compound and capsid stabilizer, was added during the induction period and during purification. Purification was by immunoaffinity chromatography. The purified empty capsids had the same immunogenicity as poliovirus virions. The techniques described might be useful for the production of new non-infectious vaccines.
- Subjects :
- 3C Viral Proteases
Antigens, Viral immunology
Antigens, Viral isolation & purification
Capsid biosynthesis
Capsid immunology
Capsid isolation & purification
Chromatography, Affinity
Cloning, Molecular
Cysteine Endopeptidases immunology
Cysteine Endopeptidases isolation & purification
Piperidines
Poliovirus immunology
Poliovirus Vaccine, Inactivated
Preservatives, Pharmaceutical
Pyridazines
Recombinant Proteins biosynthesis
Recombinant Proteins immunology
Recombinant Proteins isolation & purification
Saccharomyces cerevisiae
Viral Structural Proteins genetics
Viral Structural Proteins immunology
Viral Structural Proteins isolation & purification
Antigens, Viral biosynthesis
Cysteine Endopeptidases biosynthesis
Genes, Viral
Poliovirus genetics
Viral Proteins
Viral Structural Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 78 ( Pt 8)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 9266976
- Full Text :
- https://doi.org/10.1099/0022-1317-78-8-1829