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Endogenous glutamate levels regulate nerve growth factor mRNA expression in the rat dentate gyrus.
- Source :
-
Molecules and cells [Mol Cells] 1997 Jun 30; Vol. 7 (3), pp. 425-30. - Publication Year :
- 1997
-
Abstract
- The levels of nerve growth factor (NGF) mRNA can be regulated in vitro and in vivo in the hippocampal formation by events associated with pharmacological activation of glutamate receptors. In the present study, the level of NGF mRNA in the hippocampal formation was examined following an intrahippocampal injection of 1 nmole fluorocitrate, which temporarily inhibits the astrocyte metabolic activity in vivo. Consistent with previous findings, fluorocitrate treatment significantly increased glutamate levels and decreased glutamine levels in the dentate gyrus as determined by in vivo microdialysis. The increased ratio of glutamate to glutamine was followed by a significant increase in NGF mRNA expression selectively in dentate gyrus granule cells. The effects of increasing glutamate levels were blocked by pretreatment with 50 nmole 2-amino-5-phosphonovalerate (AP5), a competitive antagonist that acts at the N-methyl-D-aspartate (NMDA) glutamate receptor subtype. These findings suggest that NGF mRNA expression is regulated, in part, by changes in endogenous glutamate levels, partially through enhanced excitatory neurotransmission through NMDA receptors.
- Subjects :
- 2-Amino-5-phosphonovalerate pharmacology
Animals
Astrocytes drug effects
Astrocytes metabolism
Citrates pharmacology
Dentate Gyrus drug effects
Dentate Gyrus physiology
Female
Gene Expression Regulation
Glutamine metabolism
In Situ Hybridization
Microdialysis
Rats
Receptors, N-Methyl-D-Aspartate antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate metabolism
Synaptic Transmission
Dentate Gyrus metabolism
Glutamic Acid metabolism
Nerve Growth Factors genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1016-8478
- Volume :
- 7
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecules and cells
- Publication Type :
- Academic Journal
- Accession number :
- 9264033