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An altered intracellular distribution of the autoantigen La/SS-B when translated from a La mRNA isoform.
- Source :
-
Experimental cell research [Exp Cell Res] 1997 Aug 01; Vol. 234 (2), pp. 329-35. - Publication Year :
- 1997
-
Abstract
- Transcription of the gene encoding for the nuclear autoantigen La resulted in La mRNA isoforms. A promoter switching combined with an alternative splicing pathway replaced exon 1 with exon 1'. Similar to mRNAs encoding for ribosomal proteins, exon 1' started with a pyrimidine-rich 5'-terminus. Moreover, exon 1' contained 5'-GC-rich regions and an oligo(U)-tail of 23 uridine residues. Exon 1' encoded for three open reading frames upstream of the La protein reading frame. In spite of this unusual structure, exon 1' La mRNAs were translated not only in vitro but also in transiently transfected cells. The translational efficiency of exon 1' La mRNA was about 14% of exon 1 La mRNA using rabbit reticulolysate for in vitro translation. Finally, we established permanently transfected mouse cell lines expressing the human exon 1 or exon 1' La mRNA isoform. In all cell lines the respective La mRNAs were translated to La protein. The exon 1 La mRNA-expressing cell lines displayed a mostly nuclear staining pattern. In contrast, a major portion of La protein was found in the cytoplasm of cell lines expressing exon 1' La mRNA.
- Subjects :
- 3T3 Cells
Alternative Splicing
Animals
Cell Line
Cell Nucleus chemistry
Cell-Free System
DNA, Recombinant
Exons genetics
Humans
Mice
Protein Biosynthesis genetics
Rabbits
Transfection
SS-B Antigen
Autoantigens analysis
Autoantigens genetics
Cytoplasm chemistry
RNA, Messenger genetics
Ribonucleoproteins analysis
Ribonucleoproteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4827
- Volume :
- 234
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 9260901
- Full Text :
- https://doi.org/10.1006/excr.1997.3608