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Development of in vitro model systems for destructive joint diseases: novel strategies for establishing inflammatory pannus.
- Source :
-
Arthritis and rheumatism [Arthritis Rheum] 1997 Aug; Vol. 40 (8), pp. 1420-8. - Publication Year :
- 1997
-
Abstract
- Objective: To establish a novel 3-dimensional (3-D) in vitro model for the investigation of destructive processes in rheumatoid arthritis (RA).<br />Methods: Two distinct culture systems were developed, consisting of RA synovial membrane and articular cartilage explants or interactive RA synovial cell/chondrocyte cultures embedded in 3-D fibrin matrices. The expression of proteolytic enzymes, chondrocyte matrix architecture, and matrix degradation parameters was analyzed by immunohistochemistry.<br />Results: Of 28 RA explant cultures, 16 displayed an invasion of synovial tissue into the cartilage explants, compared with 1 of 8 osteoarthritis explants. The expression of collagenase and vascular cell adhesion molecule 1 could be demonstrated at the cartilage-pannus junction. Of 20 interactive cell cultures, 18 revealed invasive behavior and remained vital for extended periods of time.<br />Conclusion: The models presented allow us to study distinct aspects of destructive joint diseases under in vitro conditions that resemble human pathology. Moreover, our model is able to supplement animal experiments in basic research and drug testing.
- Subjects :
- Animals
Antigens, CD analysis
Antigens, Differentiation, Myelomonocytic analysis
Cartilage, Articular physiopathology
Cells, Cultured
Collagenases analysis
Culture Techniques
Fibroblasts chemistry
HLA-DR Antigens analysis
Humans
Immunohistochemistry
Lipopolysaccharide Receptors analysis
Macrophages immunology
Matrix Metalloproteinase 1
Proliferating Cell Nuclear Antigen analysis
Synovial Membrane cytology
Vascular Cell Adhesion Molecule-1 analysis
Arthritis, Rheumatoid physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0004-3591
- Volume :
- 40
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Arthritis and rheumatism
- Publication Type :
- Academic Journal
- Accession number :
- 9259421
- Full Text :
- https://doi.org/10.1002/art.1780400809