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Organization of the Thermus thermophilus nusA/infB operon and overexpression of the infB gene in Escherichia coli.
- Source :
-
Biochimie [Biochimie] 1997 Apr; Vol. 79 (4), pp. 195-203. - Publication Year :
- 1997
-
Abstract
- The structural gene for translation initiation factor IF2 from Thermus thermophilus was identified on the basis of the N-terminal amino acid sequence of intact T thermophilus IF2 and an internal 25 kDa IF2 fragment. A total of 5135 bp was cloned and sequenced, comprising the open reading frames for p15A, NusA, p10A, IF2, p10B and SecD, which may form an operon. There are pronounced similarities between the operon arrangement and primary sequence of the T thermophilus genes and proteins, respectively, and their counterparts from other organisms. The T thermophilus infB gene was expressed to a high level in E coli. Four hundred milligrams of homogenous T thermophilus IF2 were prepared from 60 g of overproducing cells.
- Subjects :
- Amino Acid Sequence
Bacterial Proteins biosynthesis
Bacterial Proteins isolation & purification
Base Sequence
Cloning, Molecular
DNA Primers
DNA, Bacterial genetics
Escherichia coli Proteins
Gene Expression
Molecular Sequence Data
Peptide Initiation Factors biosynthesis
Peptide Initiation Factors isolation & purification
Polymerase Chain Reaction
Prokaryotic Initiation Factor-2
Recombinant Proteins biosynthesis
Recombinant Proteins isolation & purification
Transcription Factors biosynthesis
Transcription Factors isolation & purification
Transcriptional Elongation Factors
Bacterial Proteins genetics
Escherichia coli genetics
Operon
Peptide Elongation Factors
Peptide Initiation Factors genetics
Thermus thermophilus genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0300-9084
- Volume :
- 79
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochimie
- Publication Type :
- Academic Journal
- Accession number :
- 9242984
- Full Text :
- https://doi.org/10.1016/s0300-9084(97)83506-7