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A soluble 12-kDa protein of the mitochondrial intermembrane space, Mrs11p, is essential for mitochondrial biogenesis and viability of yeast cells.
- Source :
-
Molecular & general genetics : MGG [Mol Gen Genet] 1997 Jun; Vol. 255 (2), pp. 157-65. - Publication Year :
- 1997
-
Abstract
- We have isolated an essential yeast gene termed MRS11, which codes for a soluble protein of the mitochondrial intermembrane space. Interestingly, this new gene shares many similarities with the previously characterized MRS5 gene: when expressed from a multicopy plasmid, MRS11 like MRS5 restores respiration competence to yeast strains defective in the splicing of mitochondrial group II introns. Both genes are essential for viability of yeast cells, as the disruption of either of them is lethal. The proteins encoded by MRS5 and MRS11, which display 35%, sequence identity are both located in the mitochondrial intermembrane space. Depletion of Mrs11p results in a phenotype similar to that observed in Mrs5p-depleted cells: accumulation of the precursor form of mitochondrial hsp60, inability to form spectrophotometrically detectable amounts of cytochromes and changes in the mitochondrial morphology. Although similar in sequence and function, Mrs5p and Mrs11p are not functionally equivalent and neither can substitute for the other, even when overexpressed. Taken together, our data suggest a cooperative mode of action of Mrs11p and Mrs5p in mitochondrial protein import or other related essential mitochondrial processes.
- Subjects :
- Amino Acid Sequence
Base Sequence
Biological Transport
Cell Division genetics
Chaperonin 60 metabolism
Chromosome Mapping
Cloning, Molecular
Cytochromes analysis
Cytochromes metabolism
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Lethal
Intracellular Membranes metabolism
Membrane Proteins genetics
Mitochondrial Precursor Protein Import Complex Proteins
Molecular Sequence Data
Protein Precursors metabolism
Saccharomyces cerevisiae genetics
Sequence Homology, Amino Acid
Solubility
Spectrophotometry
Fungal Proteins genetics
Fungal Proteins metabolism
Mitochondria metabolism
Saccharomyces cerevisiae Proteins
Yeasts genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0026-8925
- Volume :
- 255
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular & general genetics : MGG
- Publication Type :
- Academic Journal
- Accession number :
- 9236772
- Full Text :
- https://doi.org/10.1007/s004380050484