Back to Search
Start Over
Stromal fibroblasts influence oral squamous-cell carcinoma cell interactions with tenascin-C.
- Source :
-
International journal of cancer [Int J Cancer] 1997 Jul 17; Vol. 72 (2), pp. 369-76. - Publication Year :
- 1997
-
Abstract
- In this study we identified tenascin-C (TN-C) and one of its integrin receptors, alpha(v)beta6, in oral squamous-cell carcinoma (SCC) specimens. Neither TN-C nor alpha(v)beta6 are expressed in normal oral mucosa. We also studied 2 human oral squamous-cell carcinoma cell lines: the highly invasive HSC-3 cells, and the poorly invasive SCC-25 cells. We determined that adhesion of these cells to TN-C involves both alpha2 and alpha(v) integrins. Migration on TN-C by oral SCC cells required fibroblast-conditioned medium and did not occur in its absence. This migration was blocked by anti-alpha2 and anti-alpha(v) antibodies and was partially inhibited by antibodies to hepatocyte growth factor, epidermal growth factor and transforming growth factor-beta1. When seeded on TN-C, the poorly invasive SCC-25 cells formed alpha(v)beta6-positive focal contacts; the HSC-3 cells did not. HSC-3, SCC-25 and PTF cells secrete TN-C into the culture medium, as determined by Western blot. However, when HSC-3 cells were inoculated into the floor of the mouth of nude mice, only murine TN-C could be identified in the reactive stroma adjacent to the resulting tumor nests, demonstrating that in vivo, HSC-3 cells do not secrete TN-C. Our results demonstrate that alpha(v)beta6 and tenascin-C are neo-expressed in oral squamous-cell carcinoma, and that the tumor stromal environment is influential in oral SCC behavior.
- Subjects :
- Animals
Carcinoma, Squamous Cell pathology
Cell Adhesion
Cell Movement
Culture Media, Conditioned
Fibroblasts metabolism
Fibroblasts pathology
Humans
Integrins metabolism
Mice
Mouth Neoplasms pathology
Stromal Cells pathology
Tumor Cells, Cultured
Antigens, Neoplasm
Carcinoma, Squamous Cell metabolism
Mouth Neoplasms metabolism
Stromal Cells metabolism
Tenascin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 72
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 9219848
- Full Text :
- https://doi.org/10.1002/(sici)1097-0215(19970717)72:2<369::aid-ijc28>3.0.co;2-9