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Spectrofluorimetric determination of 5-methoxypsoralen pharmacokinetic in patients' serum.
Spectrofluorimetric determination of 5-methoxypsoralen pharmacokinetic in patients' serum.
- Source :
-
Experimental dermatology [Exp Dermatol] 1997 Apr; Vol. 6 (2), pp. 57-63. - Publication Year :
- 1997
-
Abstract
- In medicine, psoriasis and vitiligo are most often treated with PUVA therapy (psoralen plus ultraviolet A). The determination of psoralen in patients' blood is necessary, as it is admitted that the therapeutic efficiency depends on drug concentration in patients' serum. The amount of UVA to administer is inversely proportional to serum peak concentration. High-performance liquid chromatography (HPLC) and gas chromatography are the most employed methods for determining psoralens in patients' serum. The 2 techniques are precise and very sensitive, but time consuming. The aim of this paper is to propose a suitable method which is rapid and simple. It is a spectrofluorimetric technique for 5-methoxypsoralen (5-MOP) determination in the serum of patients treated with PUVA therapy. 5-MOP extraction was carried out with an heptane/dichloromethane mixture (4/1; v/v), according to the Stolk method (1980). A calibration curve (CC) was plotted from 5-MOP concentrations (range 50, 100, 200, 300, 400, 500 ng/ml). The CC was linear with a good coefficient of correlation: r = 0.9971, and a suitable coefficient of variation (CV) of 7.0%. The recovery of the method ranged from 85.3 +/- 4.2 to 108 +/- 4.1%. The assay precision gave a CV ranging from 0.10 to 6.90%, with an error inferior to +/-10%. The method did not reveal any interference from serum components on the 5-MOP emission wavelength. The limit of detection of 5-MOP was 15 ng/ml. The proposed procedure was proved to be appropriate for a rapid determination of 5-MOP in patients' serum. This technique could also be employed for other psoralens used in PUVA therapy (e.g., 8-methoxypsoralen).
- Subjects :
- 5-Methoxypsoralen
Administration, Oral
Calibration
Humans
Methanol
Methoxsalen administration & dosage
Methoxsalen blood
Methoxsalen pharmacokinetics
PUVA Therapy
Photosensitizing Agents administration & dosage
Photosensitizing Agents blood
Reproducibility of Results
Sensitivity and Specificity
Solvents
Methoxsalen analogs & derivatives
Photosensitizing Agents pharmacokinetics
Spectrometry, Fluorescence
Subjects
Details
- Language :
- English
- ISSN :
- 0906-6705
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 9209885
- Full Text :
- https://doi.org/10.1111/j.1600-0625.1997.tb00147.x