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Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy.
- Source :
-
Parasitology research [Parasitol Res] 1997; Vol. 83 (5), pp. 405-12. - Publication Year :
- 1997
-
Abstract
- Adhesion molecules play an important role in inflammatory and immunological responses. We assessed the expression pattern of intercellular adhesion molecule-1 (ICAM-1) and lymphocytefunction-associated antigen-1 (LFA-1) in the livers of mice experimentally infected with Schistosoma mansoni and in synchronous hepatic granulomas induced by injection of soluble egg antigen (SEA)-coupled Sepharose beads in a mesenteric vein of mice. By immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy, ICAM-1 was localized on endothelial cells, sinusoidal-lining cells (Kupffer cells and sinusoidal endothelium), the hepatocyte cell membrane facing Disse's space, and inflammatory cells in the granuloma. LFA-1 was visualized on the inflammatory cells of the granuloma and on phagocytic sinusoidal-lining cells, most likely Kupffer cells. ICAM-1- and LFA-1-immunoreactive cells were present in the granuloma as early as at 3 days after injection of SEA-coupled beads and persisted with time. As granulomas became older, nonimmunoreactive granuloma cells appeared. We conclude that adhesion molecules play an important role in the genesis of the schistosomal granuloma.
- Subjects :
- Animals
Disease Models, Animal
Granuloma pathology
Humans
Immunoenzyme Techniques
Liver pathology
Liver Diseases, Parasitic pathology
Male
Mice
Microscopy, Confocal
Microscopy, Immunoelectron
Schistosomiasis mansoni pathology
Granuloma metabolism
Intercellular Adhesion Molecule-1 analysis
Liver chemistry
Liver Diseases, Parasitic metabolism
Lymphocyte Function-Associated Antigen-1 analysis
Schistosomiasis mansoni metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0932-0113
- Volume :
- 83
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 9197386
- Full Text :
- https://doi.org/10.1007/s004360050273