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Chromogranin A plasma concentration and expression in pancreatic islet cell tumors of dogs and cats.
- Source :
-
American journal of veterinary research [Am J Vet Res] 1997 Jun; Vol. 58 (6), pp. 615-20. - Publication Year :
- 1997
-
Abstract
- Objectives: To describe expression of the neuroendocrine marker chromogranin A (CgA) in canine and feline pancreatic islet cell tumors and their metastases, and to evaluate plasma CgA concentration in dogs and cats with insulinoma.<br />Sample Population: Paraffin-embedded tissues from 25 canine and 2 feline pancreatic islet cell tumors, 5 canine and 6 feline exocrine pancreatic tumors, and normal pancreatic tissue from 2 dogs and 2 cats. Heparinized plasma samples from 3 dogs and 2 cats diagnosed with insulinoma, and 10 control plasma samples from each species.<br />Procedure: Immunohistochemical analysis was performed on the 42 tissue specimens, using antisera against CgA, neuron-specific enolase, insulin, somatostatin, glucagon, and pancreatic polypeptide. The 25 plasma samples were evaluated, using a soluble-phase, double-antibody, equilibrium radioimmunoassay directed against the amino- and carboxy-terminal peptides of bovine CgA.<br />Results: Chromogranin A expression was found in 76% of canine and 2 of 2 feline pancreatic islet cell tumors. Of 7 animals with CgA immunoreactivity in primary tumors, 6 also had CgA immunostaining of metastatic lesions. Plasma CgA concentration in 2 dogs with insulinoma (0.9, 1.0 ng/ml) exceeded the reference range established for 10 clinically normal control dogs (0.50 +/- 0.16 ng/ml). Feline plasma CgA samples had extensive nonspecific background immunoreactivity.<br />Conclusions: Chromogranin A is a useful immunohistochemical marker for pancreatic tumors of neuroendocrine origin and their metastases. Plasma CgA concentration determined by radioimmunoassay was high in 2 dogs with insulinoma.<br />Clinical Relevance: Immunohistochemical staining of tissues or cytologic specimens for CgA and/or neuron-specific enolase may help distinguish masses of unknown origin as neuroendocrine in nature. Increase in plasma CgA concentration may be useful diagnostically for animals with suspected neuroendocrine tumors.
- Subjects :
- Animals
Cat Diseases genetics
Cat Diseases metabolism
Cats
Cattle
Chromogranin A
Chromogranins immunology
Dog Diseases genetics
Dog Diseases metabolism
Dogs
Gene Expression Regulation, Neoplastic
Glucagon analysis
Glucagon immunology
Immune Sera immunology
Immunohistochemistry
Insulin analysis
Insulin immunology
Insulinoma blood
Insulinoma chemistry
Pancreas chemistry
Pancreas metabolism
Pancreas pathology
Pancreatic Neoplasms blood
Pancreatic Neoplasms chemistry
Pancreatic Polypeptide analysis
Pancreatic Polypeptide immunology
Phosphopyruvate Hydratase analysis
Phosphopyruvate Hydratase immunology
Radioimmunoassay methods
Radioimmunoassay veterinary
Somatostatin analysis
Somatostatin immunology
Cat Diseases blood
Chromogranins biosynthesis
Chromogranins blood
Dog Diseases blood
Insulinoma veterinary
Pancreatic Neoplasms veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9645
- Volume :
- 58
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- American journal of veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 9185968