Back to Search
Start Over
Polyvinyl alcohol-coated perfluorocarbon supports for metal chelating affinity separation of a monoclonal antibody.
- Source :
-
Journal of molecular recognition : JMR [J Mol Recognit] 1996 Sep-Dec; Vol. 9 (5-6), pp. 394-400. - Publication Year :
- 1996
-
Abstract
- The preparation, characterisation and testing of stable non-porous coated perfluorocarbon supports functionalised with the metal chelate, iminodiacetic acid (IDA) is described. Polyvinyl alcohol (PVA), a neutral hydrophilic polymer was esterified with perfluorooctanoyl chloride and anchored to the surface of solid perfluorocarbon particles through multiple fluorophilic interactions. The PVA-coated particles were then activated by epoxidation and coupled with IDA. The presence of surface-attached chelates was clearly demonstrated by the binding and selective desorption of Zn2+ ions. Three particulate perfluorocarbons were selected as potential starting materials and the conditions for preparation of metal chelating adsorbents optimised with respect to ease of manufacture, ligand density and binding capacity towards a monoclonal antibody known to bind to commercial Zn(2+)-IDA supports. The choice of base particle strongly influenced the ligand densities and specific binding capacities towards the monoclonal antibody that could be achieved under optimal preparative conditions. Possible ways in which these metal chelating adsorbents may be employed to recover the monoclonal antibody directly from culture vessels are discussed.
- Subjects :
- Animals
Butylene Glycols chemistry
CHO Cells
Cricetinae
Fluorocarbons chemistry
Metals
Sodium Hydroxide chemistry
Time Factors
Antibodies, Monoclonal isolation & purification
Chelating Agents chemistry
Chromatography, Affinity methods
Fluorocarbon Polymers chemistry
Imino Acids chemistry
Polyvinyl Alcohol chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0952-3499
- Volume :
- 9
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Journal of molecular recognition : JMR
- Publication Type :
- Academic Journal
- Accession number :
- 9174916
- Full Text :
- https://doi.org/10.1002/(sici)1099-1352(199634/12)9:5/6<394::aid-jmr273>3.0.co;2-b