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Bio-Beads: an efficient strategy for two-dimensional crystallization of membrane proteins.
- Source :
-
Journal of structural biology [J Struct Biol] 1997 Apr; Vol. 118 (3), pp. 226-35. - Publication Year :
- 1997
-
Abstract
- This work establishes the potential of Bio-Beads as a simple alternative to conventional dialysis for removing detergent and for obtaining 2D crystals of integral membrane proteins useful for structure analysis by electron crystallography. Kinetic and equilibrium aspects of removal of different detergents by adsorption onto hydrophobic Bio-Beads SM2 have been systematically investigated and extended to 2D crystallization of different prototypic membrane proteins, including: (a) Ca2+ ATPase from sarcoplasmic reticulum; (b) melibiose permease from Escherichia coli; (c) cytochrome b6f from Chlamydomonas reinhardtii. Different crystals could be produced from all protein preparations, with optical diffraction down to 20-25 A in negative stain.
- Subjects :
- Animals
Calcium-Transporting ATPases chemistry
Calcium-Transporting ATPases isolation & purification
Calcium-Transporting ATPases ultrastructure
Chlamydomonas reinhardtii chemistry
Crystallization
Cytochrome b Group chemistry
Cytochrome b Group isolation & purification
Cytochrome b Group ultrastructure
Cytochrome b6f Complex
Detergents isolation & purification
Dialysis
Escherichia coli enzymology
Membrane Proteins chemistry
Membrane Proteins ultrastructure
Membrane Transport Proteins chemistry
Membrane Transport Proteins isolation & purification
Membrane Transport Proteins ultrastructure
Microscopy, Electron
Molecular Structure
Phospholipids isolation & purification
Protein Binding
Sarcoplasmic Reticulum enzymology
Temperature
Membrane Proteins isolation & purification
Polystyrenes
Symporters
Subjects
Details
- Language :
- English
- ISSN :
- 1047-8477
- Volume :
- 118
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of structural biology
- Publication Type :
- Academic Journal
- Accession number :
- 9169232
- Full Text :
- https://doi.org/10.1006/jsbi.1997.3848