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Incorporation of [U-14C]palmitate into rat brain: effect of an inhibitor of beta-oxidation.
- Source :
-
Journal of lipid research [J Lipid Res] 1997 Feb; Vol. 38 (2), pp. 295-300. - Publication Year :
- 1997
-
Abstract
- We examined the effect of a clinically therapeutic dose of methyl 2-tetradecylglycidate (McN-3716, methyl palmoxirate, MEP) (2.5 mg/kg), an inhibitor of beta-oxidation of fatty acids, on incorporation of radiolabeled palmitic acid ([U-14C]PAM) from plasma into brain lipids of awake rats. Four hour pretreatment with 2.5 mg/kg MEP significantly increased the incorporation of [U-14C]PAM into brain lipids and substantially decreased aqueous radiolabeled metabolites in brain that can constitute unwanted background signal when analyzed by quantitative autoradiography. MEP treatment increased the lipid to aqueous background radioactivity from 0.8 to 3.0. Net rate of incorporation, k*, was significantly increased (60%) by MEP and was attributed to incorporation of [U-14C]PAM into phospholipid and triglyceride brain compartments. MEP treatment did not affect the size of the fatty acyl-CoA pool or the distribution of the various molecular acyl-CoA species. These results indicate that MEP, at a dose of 2.5 mg/kg (per os), can be used to increase incorporation of [1-(11C)]PAM for studying brain lipid metabolism in humans by positron emission tomography (PET).
- Subjects :
- Acyl Coenzyme A classification
Acyl Coenzyme A drug effects
Animals
Brain drug effects
Carbon Radioisotopes
Lipids classification
Male
Oxidation-Reduction
Palmitic Acid analysis
Phospholipids biosynthesis
Phospholipids classification
Rats
Rats, Inbred F344
Acyl Coenzyme A biosynthesis
Brain metabolism
Epoxy Compounds pharmacology
Hypoglycemic Agents pharmacology
Lipid Metabolism
Palmitic Acid metabolism
Propionates pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2275
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of lipid research
- Publication Type :
- Academic Journal
- Accession number :
- 9162749