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Isolation of the gene encoding lamp-1, a lysosomal membrane protein, by differential screening in an animal model of status epilepticus.

Authors :
Sun N
Bruce AJ
Baudry M
Schreiber SS
Source :
Brain research. Molecular brain research [Brain Res Mol Brain Res] 1997 May; Vol. 45 (2), pp. 353-5.
Publication Year :
1997

Abstract

The present study employed differential library screening to identify genes associated with kainic acid (KA)-mediated selective neuronal death. One of the isolated clones was lamp-1, which encodes a major lysosomal membrane protein that is also present in the cell membrane. Following systemic KA treatment, lamp-1 was induced in vulnerable hippocampal and other limbic regions. This effect was blocked by cycloheximide (CHX) pre-treatment. Northern blot analysis also demonstrated the presence of lamp-1 transcripts in non-neural tissues. These findings suggest a novel role for lysosomal membrane proteins as markers of selective neuronal vulnerability.

Details

Language :
English
ISSN :
0169-328X
Volume :
45
Issue :
2
Database :
MEDLINE
Journal :
Brain research. Molecular brain research
Publication Type :
Academic Journal
Accession number :
9149114
Full Text :
https://doi.org/10.1016/s0169-328x(97)00075-2