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Isolation of the gene encoding lamp-1, a lysosomal membrane protein, by differential screening in an animal model of status epilepticus.
- Source :
-
Brain research. Molecular brain research [Brain Res Mol Brain Res] 1997 May; Vol. 45 (2), pp. 353-5. - Publication Year :
- 1997
-
Abstract
- The present study employed differential library screening to identify genes associated with kainic acid (KA)-mediated selective neuronal death. One of the isolated clones was lamp-1, which encodes a major lysosomal membrane protein that is also present in the cell membrane. Following systemic KA treatment, lamp-1 was induced in vulnerable hippocampal and other limbic regions. This effect was blocked by cycloheximide (CHX) pre-treatment. Northern blot analysis also demonstrated the presence of lamp-1 transcripts in non-neural tissues. These findings suggest a novel role for lysosomal membrane proteins as markers of selective neuronal vulnerability.
- Subjects :
- Animals
Cycloheximide pharmacology
Disease Models, Animal
Hippocampus drug effects
Kainic Acid pharmacology
Limbic System drug effects
Lysosomal Membrane Proteins
Lysosomes metabolism
Male
Organ Specificity
Rats
Rats, Sprague-Dawley
Status Epilepticus chemically induced
Antigens, CD biosynthesis
Hippocampus metabolism
Limbic System metabolism
Membrane Glycoproteins biosynthesis
Pyramidal Cells metabolism
Status Epilepticus metabolism
Transcription, Genetic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0169-328X
- Volume :
- 45
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain research. Molecular brain research
- Publication Type :
- Academic Journal
- Accession number :
- 9149114
- Full Text :
- https://doi.org/10.1016/s0169-328x(97)00075-2