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Membrane fluidity and oxygen diffusion in cholesterol-enriched erythrocyte membrane.
Membrane fluidity and oxygen diffusion in cholesterol-enriched erythrocyte membrane.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 1997 May 01; Vol. 341 (1), pp. 34-9. - Publication Year :
- 1997
-
Abstract
- This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol-protein ratio (C/Pt). The membrane fluidity, as assessed by a fluorescence polarization method with diphenyl-hexatriene and 1-(4-trimethylamino)-6-phenylhexa-1,3,5-triene, decreased as the C/Pt ratio increased. Time-resolved fluorescence spectroscopy of pyrene dodecanoic acid (PDA) under an increasing C/Pt ratio in the erythrocyte membrane revealed enhanced oxygen diffusion in the middle of the membrane bilayer (in which PDA was incorporated), which was not the case with pyrene butyric acid (PBA) incorporated in the internal part of the membrane surface. It has generally been accepted that increased membrane fluidity reduces the physical barrier to oxygen permeation. Such conflicting observations on oxygen permeation in the rigidified erythrocyte membrane could be due to variations in oxygen solubility (preferential partitioning) in different polarity microdomains (cholesterol and phospholipid partitions).
- Subjects :
- Cholesterol analysis
Cholesterol Esters metabolism
Cholesterol Esters pharmacology
Diffusion
Erythrocyte Membrane chemistry
Fluorescence Polarization
Humans
Membrane Fluidity drug effects
Molecular Probes metabolism
Molecular Structure
Pyrenes metabolism
Temperature
Cholesterol physiology
Erythrocyte Membrane metabolism
Membrane Fluidity physiology
Oxygen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0003-9861
- Volume :
- 341
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 9143350
- Full Text :
- https://doi.org/10.1006/abbi.1997.9936