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Identification of direct repeat 4 as a positive regulatory element for the human TR4 orphan receptor. A modulator for the thyroid hormone target genes.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1997 May 02; Vol. 272 (18), pp. 12215-20. - Publication Year :
- 1997
-
Abstract
- While the TR4 orphan receptor (TR4) is able to repress the expression of its target genes via its interaction with the direct repeat 1-hormone response element (DR1-HRE) and DR2-HRE, we now report that TR4 can also induce the transcriptional activity of the reporter gene containing a DR4-HRE via chloramphenicol acetyltransferase assay. Electrophoretic mobility shift assay and Scatchard analysis reveal a strong binding affinity (dissociation constant = 2 nM) between TR4 and DR4-HRE. The induction mediated by TR4 was detected not only in the synthetic DR4-HRE but also in some genes, such as rat alpha-myosin heavy-chain and S14 genes, containing the DR4 or DR4-like motif, which have been suggested to be the response elements for a thyroid hormone receptor. Our data also demonstrate this TR4-mediated gene induction is TR4 dose- and DR4 sequence-dependent. Together, our data suggest that DR4-HRE can be a positive regulatory element for TR4, which may be able to induce the transcriptional activity of the genes containing such positive HREs.
- Subjects :
- Animals
Base Sequence
Binding Sites
DNA-Binding Proteins metabolism
Genes, Reporter
HIV Long Terminal Repeat
Humans
Kinetics
Mutagenesis, Site-Directed
Myosin Heavy Chains biosynthesis
Nerve Tissue Proteins biosynthesis
Protein Biosynthesis
Rats
Receptors, Steroid biosynthesis
Receptors, Thyroid Hormone biosynthesis
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins metabolism
Transfection
Tumor Cells, Cultured
Nerve Tissue Proteins metabolism
Receptors, Steroid metabolism
Receptors, Thyroid Hormone metabolism
Regulatory Sequences, Nucleic Acid
Repetitive Sequences, Nucleic Acid
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 272
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9115296
- Full Text :
- https://doi.org/10.1074/jbc.272.18.12215