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Evidence for enhanced uptake of ATP by liver and kidney in hemorrhagic shock.
- Source :
-
The American journal of physiology [Am J Physiol] 1977 Sep; Vol. 233 (3), pp. R83-8. - Publication Year :
- 1977
-
Abstract
- It has been shown that infusion of ATP-MgCl2 proved beneficial in the treatment of shock; however, it is not known whether this effect is due to improvement in the microcirculation or direct provision of energy or a combination of the above or other effects. To elucidate the mechanism of the salutary effect of ATP-MgCl2, we have now examined the in vitro uptake of ATP by liver and kidney of animals in shock. Rats were bled to a mean arterial pressure of 40 Torr and so maintained for 2 hrs. After the rats were killed, liver and kidney were removed and slices of tissue (0.3-0.5 mm thick) were incubated for 1 h in 1.0 ml of Krebs-HCO3 buffer containing 10 mM glucose, 5 mM MgCl2, and 5 mM [8-14C]ATP or 5 mM [8-14C]ADP, or 5 mM [8-14C]AMP, or 5 mM [8-14C]adenosine in 95% O2-5% CO2 and then homogenized. Tissue and medium samples were subjected to electrophoresis to separate and measure the various nucleotides. The uptake of [14C]ATP but not that of [14C]ADP or [14C]adenosine by liver and kidney slices from animals in shock was 2.5 times greater than the corresponding uptake by control slices. Thus, the beneficial effect of ATP-MgCl2 in shock could be due to provision of energy directly to tissue in which ATP levels were lowered.
- Subjects :
- Adenosine metabolism
Adenosine Diphosphate metabolism
Adenosine Monophosphate metabolism
Adenosine Triphosphate therapeutic use
Animals
Inosine metabolism
Magnesium therapeutic use
Rats
Shock, Hemorrhagic therapy
Adenosine Triphosphate metabolism
Kidney metabolism
Liver metabolism
Shock, Hemorrhagic metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 233
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 910939
- Full Text :
- https://doi.org/10.1152/ajpregu.1977.233.3.R83