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A molecular analysis of Greek and UK Haemophilus influenzae conjugative resistance plasmids.
- Source :
-
The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 1997 Mar; Vol. 39 (3), pp. 303-7. - Publication Year :
- 1997
-
Abstract
- Antibiotic resistance in Haemophilus influenzae has been associated with the presence of large, chromosomally integrated, conjugative plasmids. The plasmids of 10 beta-lactamase-positive, ampicillin-resistant strains, two from the UK and eight from Greece, were investigated. Plasmids were detected and isolated after transfer to a rec-deficient recipient. Purified whole plasmid was used as probe. In addition a 12 kb PstI fragment containing the putative point of recircularization in one plasmid, p1056, was cloned and used as a probe. All plasmids shared a high degree of sequence homology suggesting that plasmids of diverse geographical origin are highly related. All plasmids also shared sequence homology with the 12 kb PstI fragment containing the point of recircularization, suggesting that the sequences involved in excision and recircularization are conserved.
- Subjects :
- Ampicillin Resistance genetics
Blotting, Southern
Cloning, Molecular
DNA, Bacterial isolation & purification
Greece
Haemophilus influenzae enzymology
Nucleic Acid Hybridization
Sequence Homology, Nucleic Acid
United Kingdom
beta-Lactamases genetics
Conjugation, Genetic genetics
Haemophilus influenzae genetics
Plasmids genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0305-7453
- Volume :
- 39
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of antimicrobial chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 9096178
- Full Text :
- https://doi.org/10.1093/jac/39.3.303