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Sodium pump and Na+/H+ activities in uremic erythrocytes. A microcalorimetric and pH-metric study.

Authors :
Kovacic H
Gallice P
Brunet P
Berland Y
Crevat A
Source :
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 1997 Mar 18; Vol. 259 (1-2), pp. 31-40.
Publication Year :
1997

Abstract

The sodium pump and Na+/H+ antiport activities in red blood cells from uremic hemodialyzed patients were measured concomitantly. The patients selected (n = 35) were normotensive and free of intercurrent illness known to affect Na transport. The Na pump activity of intact red blood cells in suspension in their own plasma was measured by flow microcalorimetry. The Na+/H+ antiport activity of the erythrocytes from the same patients was determined by a titrimetric technique. The mean global value of the sodium pump was lower in uremics than in controls (13.3 +/- 0.6 vs. 11.3 +/- 0.8 mW/l cells, P < 0.05). The Na+/H+ antiport maximal activity was decreased in uremics (2.9 +/- 0.3 vs. 4.6 +/- 0.5 mmol H+/l cells/h, P < 0.05). Our results thus confirm that uremia per se can affect sodium transport. Moreover it has been shown that a decrease in Na+/H+ antiport activity is able to counteract an impairment of sodium pump. The decrease found in this study could thus explain, at least in part, the absence of hypertension in the patients studied despite their decreased sodium pump activity.

Details

Language :
English
ISSN :
0009-8981
Volume :
259
Issue :
1-2
Database :
MEDLINE
Journal :
Clinica chimica acta; international journal of clinical chemistry
Publication Type :
Academic Journal
Accession number :
9086292
Full Text :
https://doi.org/10.1016/s0009-8981(96)06460-1