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Detection of neutral protease (Periocheck) and BANA hydrolase (Perioscan) compared with traditional clinical methods of diagnosis and monitoring of chronic inflammatory periodontal disease.
- Source :
-
Journal of clinical periodontology [J Clin Periodontol] 1997 Feb; Vol. 24 (2), pp. 110-4. - Publication Year :
- 1997
-
Abstract
- Perioscan requires a plaque sample to detect the presence of enzymes capable of degrading N-benzoyl-DL-arginine-2-naphthylamide (BANA) from relatively few anaerobic periodontal pathogens. Periocheck assays the presence of neutral proteases in crevicular fluid. The aim of this study was to compare these test kits with traditional clinical methods of detecting periodontal disease and to monitor the ability of the kits to reflect the response to initial therapy. 19 patients with moderately severe chronic periodontitis were seen before and after a course of oral hygiene and root instrumentation consisting of 4 appointments. Clinical measurements and test assays were collected at 5 diseased sites and 2 healthy sites in each subject. Complete data from 125 sites were available for statistical analysis. At baseline Periocheck had a sensitivity of 88% and a specificity of 61% whereas Perioscan had a sensitivity of 99% and a specificity of 55%, when related to the clinical diagnosis. A composite clinical assessment, based on improvement or deterioration of one whole unit change of the subjective clinical indices and 2 mm changes or greater in probing depth or probing attachment level, revealed 75 sites which improved following treatment, whereas 45 sites did not change and 5 sites deteriorated. The probability that the tests agreed with the clinical outcome after treatment, was calculated as 50.4% for Periocheck and 52% for Perioscan. The diagnostic kits did not reliably reflect the clinical assessment of periodontal disease in the cross sectional study, or the outcome following treatment.
- Subjects :
- Adult
Aged
Benzoylarginine-2-Naphthylamide
Chronic Disease
Cross-Sectional Studies
Dental Plaque enzymology
Dental Plaque Index
Dental Scaling
Female
Gingival Crevicular Fluid enzymology
Humans
Longitudinal Studies
Male
Middle Aged
Observer Variation
Periodontal Index
Periodontitis therapy
Predictive Value of Tests
Prospective Studies
Reagent Strips
Reproducibility of Results
Sensitivity and Specificity
Clinical Enzyme Tests
Peptide Hydrolases analysis
Periodontitis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0303-6979
- Volume :
- 24
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of clinical periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 9062857
- Full Text :
- https://doi.org/10.1111/j.1600-051x.1997.tb00475.x