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Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles.
- Source :
-
Human gene therapy [Hum Gene Ther] 1997 Mar 01; Vol. 8 (4), pp. 453-65. - Publication Year :
- 1997
-
Abstract
- The expanding use of adenoviral vectors for gene therapy has brought about the need for new analytical tools. We have developed an anion-exchange high-performance liquid chromatography method to analyze recombinant adenovirus serotype 5 samples. Before this assay, available analytical methods consisted of either long-term biological assays or required highly purified test articles. These methods were inadequate for optimizing adenovirus production and purification. This assay can quantitate viral particles in either crude lysates or highly pure samples. It can be used to assess particles in both dilute and concentrated samples over a wide dynamic range. Moreover, the population of viral particles eluted in the peak contains most of the infectious virions. This assay is a sensitive technique that overcomes the limitations of previous methods. It provides an essential tool to accomplish process optimization.
- Subjects :
- Adenoviridae genetics
Blotting, Western
Cell Line
Defective Viruses genetics
Electrophoresis, Polyacrylamide Gel
Evaluation Studies as Topic
Genetic Vectors
Ultracentrifugation
Adenoviridae isolation & purification
Adenoviridae metabolism
Chromatography, High Pressure Liquid methods
DNA, Viral isolation & purification
Defective Viruses isolation & purification
Defective Viruses metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1043-0342
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Human gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 9054520
- Full Text :
- https://doi.org/10.1089/hum.1997.8.4-453