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The influence of surgical preparation on cyclic nucleotide synthesis in an organ culture of human saphenous vein.
- Source :
-
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery [Eur J Vasc Endovasc Surg] 1997 Jan; Vol. 13 (1), pp. 72-8. - Publication Year :
- 1997
-
Abstract
- Objectives: To investigate adenosine cyclic 3'5' monophosphate (cAMP) and guanosine cyclic 3'5' monophosphate (cGMP) synthesis in freshly isolated and surgically prepared human saphenous vein before and after culture.<br />Setting: Bristol Heart Institute, Bristol, U.K.<br />Methods: Freshly isolated and surgically prepared human saphenous vein was obtained from patients undergoing coronary artery bypass graft surgery. cAMP and cGMP synthesis, was assessed by radioimmunoassay in response to specific simulators in segments of saphenous veins after collection and following 14 days culture.<br />Results: Immediately after collection there was a significant reduction in the synthesis of cAMP (forskolin and prostaglandin E1-stimulated) and cGMP (sodium nitroprusside-stimulated) in surgically prepared compared to freshly isolated saphenous veins. In contrast, following 14 days in culture, cAMP and cGMP synthesis was significantly elevated in surgically prepared compared to freshly isolated saphenous veins.<br />Conclusions: These data indicate that surgical preparation results in a marked reduction in cyclic nucleotide synthesis in saphenous vein which may be relevant to the pathophysiology of early vein graft failure. The normalisation of both cAMP and cGMP synthesis in surgically prepared veins following 14 days culture indicates that cyclic nucleotide synthesising capacity may not be a major determinant of neointima formation in this experimental model.
- Subjects :
- Acetylcholine pharmacology
Adult
Aged
Alprostadil pharmacology
Calcimycin pharmacology
Colforsin pharmacology
Coronary Artery Bypass
DNA analysis
Humans
Middle Aged
Organ Culture Techniques
Radioimmunoassay
Saphenous Vein drug effects
Saphenous Vein metabolism
Tunica Intima metabolism
Tunica Intima pathology
Cyclic AMP biosynthesis
Cyclic GMP biosynthesis
Saphenous Vein surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1078-5884
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 9046918
- Full Text :
- https://doi.org/10.1016/s1078-5884(97)80054-8