Back to Search
Start Over
Ribosomal proteins of mouse kidney: normal status and during compensatory renal hypertrophy.
- Source :
-
Molecular and cellular biochemistry [Mol Cell Biochem] 1977 Aug 19; Vol. 17 (1), pp. 25-30. - Publication Year :
- 1977
-
Abstract
- Ribosomes were isolated from normal and growing kidney and the protein complement was examined by a two-dimensional gel electrophoretic procedure. Proteins were resolved in the first dimension on the basis of charge and, in the second dimension, on the basis of molecular weight. 60S and 40S ribosomal subunits from normal kidney contained respectively 42 and 31 proteins. 80S ribosomes contained 23 proteins not found with either sub-unit. Nineteen of these proteins were removed from the ribosomes when isolated ribosomes were washed in a high salt buffer. Six proteins of the 80S ribosome corresponded to proteins associated with both sub-units. 80S ribosomal proteins were also studied during compensatory renal hypertrophy after 4-96 h of induced growth. The protein complement displayed by electrophoresis was identical to the pattern seen from normal renal cells.
Details
- Language :
- English
- ISSN :
- 0300-8177
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 904616
- Full Text :
- https://doi.org/10.1007/BF01732551