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Radiation protection by alpha-methyl-homocysteine thiolactone in vitro.
- Source :
-
Life sciences [Life Sci] 1997; Vol. 60 (6), pp. 341-50. - Publication Year :
- 1997
-
Abstract
- The radiation protective effect of thiol compounds is unequivocal and their use is only limited by their toxic effects. We used the principle of alpha alkylation, which renders amino acids unmetabolizable, to reduce the toxicity of homocysteine. This product, alpha-methyl-homocysteine thio-lactone, was tested for toxicity and radiation protective effect along with known protectors L-cysteine, cysteamine and WR 1065 in cell culture using V79-4 Chinese hamster lung cells. The three-day growth curve assays, useful to measure overall effects on cell growth, revealed lowest toxicity for alpha-methyl-homocysteine thiolactone (GL-2). Clonogenic survival tests, used to evaluate the retention of reproductive integrity, were carried out and revealed that GL-2 had no adverse effects in this test system. Radiation protection tests showed that GL-2 exhibited protective activity against radiation induced lethality above that seen with cysteine and cysteamine, but below WR 1065. However, GL-2 showed little or no negative effects toward the cell itself, in direct contrast to WR 1065. Our findings show a potentially important tool and principle to reduce toxicity of radiation protectors with analogous structures.
- Subjects :
- Animals
Cell Division drug effects
Cell Line
Cell Survival drug effects
Cell Survival radiation effects
Cesium Radioisotopes
Cricetinae
Cricetulus
Cysteamine pharmacology
Cysteine pharmacology
Homocysteine pharmacology
Homocysteine toxicity
Mercaptoethylamines pharmacology
Radiation Dosage
Radiation-Protective Agents toxicity
Homocysteine analogs & derivatives
Radiation, Ionizing
Radiation-Protective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 60
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 9031679
- Full Text :
- https://doi.org/10.1016/s0024-3205(96)00515-2