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Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with atmosphere.
- Source :
-
The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 1996 Dec; Vol. 28 (12), pp. 1381-91. - Publication Year :
- 1996
-
Abstract
- To study liver functions of chicken, we examined the primary culture of chicken hepatocytes, and found an easy method of long-term culture with free atmosphere exchange. Chicken hepatocytes were obtained by collagenase perfusion and cultured at 37 degrees C as a monolayer without substratum in serum-free L-15 medium (pH 7.8) with free atmosphere exchange. The amounts of albumin and transferrin in medium were assayed by ELISA. The culture of chicken hepatocytes was maintained in the serum-free L15-medium )pH 7.) and 37 degrees C with free atmosphere exchange for 20 days. The amount of albumin secreted in the medium decreased to low levels early in culture; however, this was followed by marked increase from day 9 to day 17 of culture. The amount of transferrin was constant until day 6, then it too increased with further culture. We reported an easy method for the simple monolayer culture of chicken hepatocytes in serum-free L12 medium (pH 7.8) with free atmosphere exchange over an extended period. Expression of liver-specific functions, viz. albumin and transferrin synthesis, was observed after 1 week of culture.
- Subjects :
- Air
Animals
Antibody Specificity
Cells, Cultured
Chickens
Culture Media, Serum-Free
Hydrogen-Ion Concentration
Liver cytology
Serum Albumin biosynthesis
Serum Albumin immunology
Time Factors
Transferrin biosynthesis
Transferrin immunology
Liver metabolism
Serum Albumin metabolism
Transferrin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1357-2725
- Volume :
- 28
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The international journal of biochemistry & cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 9022295
- Full Text :
- https://doi.org/10.1016/s1357-2725(96)00076-3