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Antigenic and molecular analyses of the variability of bovine respiratory syncytial virus G glycoprotein.
- Source :
-
The Journal of general virology [J Gen Virol] 1997 Feb; Vol. 78 ( Pt 2), pp. 359-66. - Publication Year :
- 1997
-
Abstract
- Antigenic variation among eight bovine respiratory syncytial virus (BRSV) isolates was determined using monoclonal antibodies (MAbs) specific for the attachment (G) protein. Two major (and one intermediate) subgroups were identified, as well as one strain that did not fit any pattern. The subgroups could also be differentiated on the basis of the Mr of the F protein cleavage product, F2. The nucleotide sequence of the G gene of seven of the BRSV strains was determined and compared with published G gene sequences. Subgroups A and A/B were more closely related in protein sequence than subgroups A and B or subgroups A/B and B. These results could not be correlated with those obtained by the determination of the Mr of the F2 polypeptide. Multiple sequence alignments showed a high level of amino acid identity at the inter-subgroup level (85% identity between subgroup A and subgroup B strains), similar to the intra-subgroup human (H)RSV identity, suggesting that the BRSV isolates form a continuum rather than distinct subgroups. However, unusual variability was observed within the immunodominant domain (amino acids 174-188) in contrast with the situation in HRSV strains belonging to the same subgroup.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Monoclonal immunology
Antibodies, Viral immunology
Antigenic Variation
Antigens, Viral genetics
Cattle
Cell Line
Chlorocebus aethiops
Cloning, Molecular
Molecular Sequence Data
Radioimmunoprecipitation Assay
Respiratory Syncytial Virus, Bovine classification
Respiratory Syncytial Virus, Bovine genetics
Sequence Homology, Amino Acid
Serotyping
Vero Cells
Viral Envelope Proteins
Viral Proteins genetics
Antigens, Viral immunology
HN Protein
Respiratory Syncytial Virus, Bovine immunology
Viral Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 78 ( Pt 2)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 9018058
- Full Text :
- https://doi.org/10.1099/0022-1317-78-2-359