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A simplified procedure for the isolation of bile acids from serum based on a batch extraction with the non-ionic resin--Amberlite XAD-7.
- Source :
-
Annals of clinical biochemistry [Ann Clin Biochem] 1977 Jul; Vol. 14 (4), pp. 235-9. - Publication Year :
- 1977
-
Abstract
- A simple and reproducible method using the non-ionic resin, Amberlite XAD-7, for the isolation of bile acids from serum by a batch procedure is described. Recoveries were greater than 95% for the non-sulphated bile acids and greater than 70% for the sulphate esters of bile acids. By using 1 g of resin, recoveries were independent of the mass (0.1-5 mumol) of the bile acid present. Up to 35 samples a day can be extracted without requiring dedication of the operator. When serum extracts were analysed by the 3alpha-hydroxysteroid dehydrogenase procedure for estimation of bile acids, virtually all the background fluorescence was eliminated. These extracts were also suitable for gas liquid chromatography, thin layer chromatography, and radioimmunoassay.
- Subjects :
- Bile Acids and Salts isolation & purification
Chenodeoxycholic Acid analogs & derivatives
Chenodeoxycholic Acid blood
Cholic Acids blood
Deoxycholic Acid blood
Glycocholic Acid analogs & derivatives
Glycocholic Acid blood
Glycocholic Acid isolation & purification
Humans
Hydroxysteroid Dehydrogenases
Lithocholic Acid analogs & derivatives
Lithocholic Acid blood
Methods
Resins, Plant
Taurocholic Acid blood
Bile Acids and Salts blood
Subjects
Details
- Language :
- English
- ISSN :
- 0004-5632
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Annals of clinical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 900857
- Full Text :
- https://doi.org/10.1177/000456327701400163