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Differential susceptibility to HIV-GP120-sensitized apoptosis in CD4+ T-cell clones with different T-helper phenotypes: role of CD95/CD95L interactions.
- Source :
-
Blood [Blood] 1997 Jan 15; Vol. 89 (2), pp. 558-69. - Publication Year :
- 1997
-
Abstract
- The susceptibility of Th1 and Th2 cell clones to apoptosis following HIV-gp120/CD4 cross-linking and TCR activation was investigated. We show that only Th1 clones are susceptible to HIV-gp120-sensitized apoptosis, although both types of clones express similar levels of CD4 and bind similar amounts of recombinant gp120. Both types of clones, however, undergo apoptosis induced by CD95 cross-linking with agonistic monoclonal antibody (MoAb). Apoptosis induced by gp120 in the Th1 clones is inhibited by either an anti-CD95 neutralizing MoAb or an anti-CD95L neutralizing MoAb as well as by a specific interleukin-1 beta converting enzyme (ICE) inhibitor. When triggered to apoptosis by gp120, Th1 but not Th2 clones express both cell-associated and soluble CD95L. The CD95L produced by Th1 clones induces cell death, inhibitable by anti-CD95 neutralizing MoAb, of CD95 positive Jurkat cells. These data suggest that, like activation-induced apoptosis, HIV-gp120 sensitized apoptosis in Th1 clones occurs via CD95/CD95L interaction and that lack or insufficient production of CD95L is responsible, at least in part, for the resistance of Th2 clones to such apoptosis.
- Subjects :
- CD4-Positive T-Lymphocytes immunology
Cells, Cultured
Fas Ligand Protein
Flow Cytometry
Humans
Immunophenotyping
Lymphocyte Activation
T-Lymphocytes, Helper-Inducer pathology
Apoptosis immunology
CD4-Positive T-Lymphocytes pathology
HIV Envelope Protein gp120 immunology
HIV-1 immunology
Membrane Glycoproteins immunology
T-Lymphocytes, Helper-Inducer immunology
fas Receptor immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 89
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 9002959