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Methylation of the 5' CpG island of the endothelin B receptor gene is common in human prostate cancer.

Authors :
Nelson JB
Lee WH
Nguyen SH
Jarrard DF
Brooks JD
Magnuson SR
Opgenorth TJ
Nelson WG
Bova GS
Source :
Cancer research [Cancer Res] 1997 Jan 01; Vol. 57 (1), pp. 35-7.
Publication Year :
1997

Abstract

Production of the potent vasoconstrictor endothelin-1 (ET-1) by human prostate cancer cells accompanies prostate cancer progression in vivo. The predominant endothelin receptor expressed by normal prostate epithelium, ETB, is not expressed by any of the established human prostate cancer cell lines, and ETB binding is decreased on prostate cancer tissues. ETB, which may mediate ET-1 clearance and may inhibit ET-1 secretion, is encoded by a gene that contains a 5' CpG island encompassing the transcriptional regulatory region. We examined this regulatory region of the ETB receptor gene (EDNRB) to determine whether hypermethylation of cytidine nucleotides accompanies decreased ETB expression in human prostate cancer. We found somatic methylation of CpG island sequences in EDNRB in 5 of 5 human prostate cancer cell lines, 15 of 21 primary prostate cancer tissues, and 8 of 14 prostate cancer metastases (70% of samples overall). Normal tissues contained only unmethylated EDNRB. Treatment of human prostatic carcinoma cell line cultures with 5-azacytidine induced ETB mRNA expression, suggesting that CpG island methylation changes might accompany the apparent transcriptional silencing of EDNRB in vivo.

Details

Language :
English
ISSN :
0008-5472
Volume :
57
Issue :
1
Database :
MEDLINE
Journal :
Cancer research
Publication Type :
Academic Journal
Accession number :
8988036