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cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen.
- Source :
-
FEBS letters [FEBS Lett] 1996 Dec 16; Vol. 399 (3), pp. 295-8. - Publication Year :
- 1996
-
Abstract
- A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature processed protein of 102 aa with a molecular mass of 11344 Da. The expressed recombinant protein, named rPar j 2.0101, was a major allergen since it reacted with IgE of 82% (23/28) of the sera of Pj-allergic subjects analyzed. It was shown to be a new allergen since (i) the amino acid sequence homology with the already reported recombinant allergen Par j 1.0101 was 45% and (ii) there was no cross-inhibition between rPar j 2.0101 and rPar j 1.0101. In addition, rPar j 2.0101 inhibited 35% of the specific IgE for 10-14 kDa native allergens and preincubation of sera from Pj-allergic patients with both rPar j 2.0101 and rPar j 1.0101 fully abolished the IgE recognition of the 10-14 kDa native allergen region, suggesting that these two allergens contributed to the region.
- Subjects :
- Allergens chemistry
Allergens immunology
Amino Acid Sequence
Antigens, Plant
Base Sequence
Cloning, Molecular
DNA, Complementary
Humans
Hypersensitivity blood
Molecular Sequence Data
Molecular Weight
Open Reading Frames
Plant Proteins chemistry
Plant Proteins immunology
Protein Sorting Signals chemistry
Protein Sorting Signals genetics
Sequence Homology, Amino Acid
Allergens genetics
Plant Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 399
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 8985165
- Full Text :
- https://doi.org/10.1016/s0014-5793(96)01328-2