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Nuclear antigens, as DNA, of DSB389 MAb and some other anti-desmin monoclonal antibodies.
- Source :
-
Cell biology international [Cell Biol Int] 1996 Nov; Vol. 20 (11), pp. 769-75. - Publication Year :
- 1996
-
Abstract
- The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were searched for in an IFs-deficient clone of SW13 cells. Reactive materials specific to DSB389, AC54, and DSB860 MAbs were trapped at the top of the gel of SDS-agarose-PAGE. The reactivity of the materials disappeared after treatment with DNase I. The reactivity, or trapping of this material at the top of the gel, required previous heat treatment of the sample before application to the gel. The MAbs recognized both single-stranded and double-stranded DNA in ELISA. These results indicate that at least the main nuclear antigens of DSB389, DSB860 and AC54 MAbs are DNA.
- Subjects :
- Adenocarcinoma immunology
Adenocarcinoma pathology
Antibody Specificity
Antigens, Neoplasm immunology
Antigens, Nuclear
Cetrimonium
Cetrimonium Compounds pharmacology
DNA isolation & purification
DNA, Neoplasm immunology
DNA, Single-Stranded isolation & purification
Detergents pharmacology
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
HeLa Cells drug effects
HeLa Cells immunology
Humans
Immunoblotting
Intermediate Filaments immunology
Intermediate Filaments ultrastructure
Nuclear Proteins isolation & purification
Octoxynol pharmacology
Tumor Cells, Cultured drug effects
Tumor Cells, Cultured immunology
Antibodies, Monoclonal immunology
DNA immunology
DNA, Single-Stranded immunology
Desmin immunology
Nuclear Matrix immunology
Nuclear Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1065-6995
- Volume :
- 20
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell biology international
- Publication Type :
- Academic Journal
- Accession number :
- 8979370
- Full Text :
- https://doi.org/10.1006/cbir.1996.0099