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Elimination of hepatitis C virus RNA in infected human hepatocytes by adenovirus-mediated expression of ribozymes.
- Source :
-
Journal of virology [J Virol] 1996 Dec; Vol. 70 (12), pp. 8782-91. - Publication Year :
- 1996
-
Abstract
- Hepatitis C virus (HCV), a positive-strand RNA virus, is the major infectious agent responsible for causing chronic hepatitis. Currently, there is no vaccine for HCV infection, and the only therapy for chronic hepatitis C is largely ineffective. To investigate new genetic approaches to the management of HCV infection, six hammerhead ribozymes directed against a conserved region of the plus strand and minus strand of the HCV genome were isolated from a ribozyme library, characterized, and expressed from recombinant adenovirus vectors. The expressed ribozymes individually or in combination were efficient at reducing or eliminating the respective plus- or minus-strand HCV RNAs expressed in cultured cells and from primary human hepatocytes obtained from chronic HCV-infected patients. This study demonstrates the potential utility of ribozyme therapy as a strategy for the treatment of hepatitis C virus infection.
- Subjects :
- Animals
Base Sequence
CHO Cells
Cells, Cultured
Cricetinae
Gene Expression
Hepatitis C pathology
Humans
Liver pathology
Liver virology
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Catalytic genetics
RNA, Catalytic isolation & purification
Recombinant Fusion Proteins
Adenoviridae genetics
Genetic Vectors
Hepacivirus genetics
Hepatitis C virology
RNA, Catalytic metabolism
RNA, Viral metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 70
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 8971007
- Full Text :
- https://doi.org/10.1128/JVI.70.12.8782-8791.1996