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Inhibition of choline incorporation into brain lipids in rats by urethane, a proposed mechanism of depression of the central nervous system.
- Source :
-
Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 1977 Jul; Vol. 298 (3), pp. 205-10. - Publication Year :
- 1977
-
Abstract
- Concentrations and specific radioactivities of choline, acetylcholine, phosphorylcholine, lipid choline, and sn-glycero-3-phosphorylcholine after i.v. injection of methyl-14C-choline were measured in the brain of untreated controls and of rats anesthesized with urethane. The specific activity was found to be decreased during deep anesthesia by 40% in acetylcholine, 20-30% in phosphorylcholine, 50-75% in lipid choline, and 30-40% in sn-glycero-3-phosphoryl-choline. No significant change was detected in the specific activity of choline. The brain concentration of acetylcholine was increased by 40%, the concentration of sn-glycero-3-phosphorylcholine, however, was diminished by 10% during anesthesia. No change was found in the concentration of the other choline containing compounds investigated. Measuring choline incorporation into 4 subcellular fractions of brain tissue specific activities were found to be decreased by the same percentage, although 2 fractions (nuclei and microsomes) were higher labelled than the 2 other fractions (crude mitochondria with synaptosomes and lysosomes). A correlation between the biochemical and the functional alterations is supported by the dose-effect relationships on both parameters. It is suggested that urethane reduces turnover of lipids and by that mechanism inhibits the exocytotic release of the transmitter from presynaptic nerve endings.
- Subjects :
- Animals
Brain drug effects
Brain ultrastructure
Chromatography, Paper
Depression, Chemical
Dose-Response Relationship, Drug
Hydrolysis
In Vitro Techniques
Male
Rats
Subcellular Fractions drug effects
Subcellular Fractions metabolism
Time Factors
Brain metabolism
Choline metabolism
Lipid Metabolism
Urethane pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0028-1298
- Volume :
- 298
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Naunyn-Schmiedeberg's archives of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 895894
- Full Text :
- https://doi.org/10.1007/BF00500888