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Inducible and endothelial nitric oxide synthase expression during development of transplant arteriosclerosis in rat aortic grafts.
- Source :
-
The American journal of pathology [Am J Pathol] 1996 Dec; Vol. 149 (6), pp. 1981-90. - Publication Year :
- 1996
-
Abstract
- In the vascular system, distinct isoforms of nitric oxide synthase (NOS) generate nitric oxide (NO), which acts as a biological messenger. Its role in the development of transplant arteriosclerosis (TA) is still unclear. To investigate whether NO is involved in TA, we studied the expression of NOS isoforms, inducible NOS (iNOS) and endothelial NOS (eNOS), by immunohistochemistry and in situ hybridization during the first two post-transplantation months and their relation with cold ischemia (1 to 24 hours) and reperfusion injury using an aortic transplantation model in the rat. We found an increased iNOS expression in the intima and adventitia and a decreased expression in the media, whereas eNOS expression was not significantly altered during the development of TA. Co-localization studies suggested that iNOS-positive cells were vascular smooth muscle cells, monocyte-derived macrophages, and endothelial cells. Prolonged ischemic storage time resulted in an increase in eNOS expression in the neointima. In situ hybridization showed iNOS mRNA expression by vascular cells in the neointima and media. NO produced by iNOS and eNOS may be involved, at least in part, in the pathogenesis of TA in aortic grafts. Additional studies are needed to confirm the modulatory mechanism of NO during the development of TA.
- Subjects :
- Amino Acid Sequence
Animals
Aorta pathology
Arteriosclerosis pathology
Endothelium, Vascular pathology
Enzyme Induction
Immunohistochemistry
Isoenzymes biosynthesis
Isoenzymes genetics
Isoenzymes immunology
Male
Molecular Sequence Data
Nitric Oxide Synthase genetics
Nitric Oxide Synthase immunology
RNA, Messenger biosynthesis
Rats
Rats, Inbred Strains
Reperfusion Injury enzymology
Reperfusion Injury pathology
Tissue Distribution
Transplantation, Homologous adverse effects
Transplantation, Isogeneic adverse effects
Aorta enzymology
Aorta transplantation
Arteriosclerosis enzymology
Arteriosclerosis etiology
Endothelium, Vascular enzymology
Nitric Oxide Synthase biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9440
- Volume :
- 149
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 8952533