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Thiamine corrects delayed replication and decreases production of lactate and advanced glycation end-products in bovine retinal and human umbilical vein endothelial cells cultured under high glucose conditions.
- Source :
-
Diabetologia [Diabetologia] 1996 Nov; Vol. 39 (11), pp. 1263-8. - Publication Year :
- 1996
-
Abstract
- This study aimed at verifying whether thiamine, a co-enzyme which decreases intracellular glycolysis metabolites by allowing pyruvate and glyceraldheyde 3-phosphate to enter the Krebs cycle and the pentose-phosphate shunt, respectively, corrects delayed replication caused by high glucose concentrations in cultured human umbilical vein (HUVEC) and bovine retinal endothelial cells (BREC). After incubation in physiological (5.6 mmol/l) or high (28.0 mmol/l) glucose with or without 150 mumol/l thiamine, cells were counted and proliferation assessed by mitochondrial dehydrogenase activity. Lactate was measured in both cell types as an index of glycolytic activity and fluorescent advanced glycosylation end-products (AGE) concentration was determined in the HUVEC lysate. Both cell counts and proliferation assays in either of the cell types confirmed the impairment to cell replication induced by high glucose. When thiamine was added to cells kept under high glucose conditions, the number of surviving cells was significantly increased and the reduced cell proliferation appeared to be corrected. Lactate assays confirmed the increased production of this metabolite by BREC and HUVEC in high glucose, which was reduced by thiamine. Fluorescent AGE determination showed that thiamine may prevent non-enzymatic glycation in HUVEC. Thiamine restores cell replication, decreases the glycolytic flux and prevents fluorescent AGE formation in endothelial cells cultured in high glucose, suggesting that abnormal levels of glycolytic metabolite(s) may damage cells.
- Subjects :
- Animals
Cattle
Cell Count drug effects
Cell Division drug effects
Cell Division physiology
Cells, Cultured
Diabetes Mellitus metabolism
Endothelium cytology
Endothelium drug effects
Endothelium metabolism
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Glycosylation drug effects
Humans
Lactic Acid metabolism
Retina cytology
Retina drug effects
Spectrometry, Fluorescence
Spectrophotometry
Umbilical Veins cytology
Endothelium, Vascular metabolism
Glucose
Glycation End Products, Advanced biosynthesis
Lactic Acid biosynthesis
Retina metabolism
Thiamine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0012-186X
- Volume :
- 39
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Diabetologia
- Publication Type :
- Academic Journal
- Accession number :
- 8932990
- Full Text :
- https://doi.org/10.1007/s001250050568