Back to Search
Start Over
Expression cloning of cDNA by phage display selection.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1996 Nov 01; Vol. 24 (21), pp. 4367-8. - Publication Year :
- 1996
-
Abstract
- Expression cloning of a mouse kappa chain fragment has been achieved from a cDNA library by display of expressed proteins on filamentous phage and affinity selection for binding to anti-mouse Fab antibodies. Expressed proteins were anchored to the phage coat by a synthetic, anti-parallel leucine zipper, which had been selected from a semi-randomized zipper library for the ability to connect a test protein to phage. From a library of 4 x 10(6) transformants, two separate clones displaying different size cDNA inserts were recovered after four selection rounds. These results further demonstrate the utility of phage display for cDNA expression cloning.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
DNA, Complementary
Immunoglobulin Fab Fragments immunology
Immunoglobulin kappa-Chains biosynthesis
Immunoglobulin kappa-Chains immunology
Leucine Zippers genetics
Mice
Molecular Sequence Data
Bacteriophages genetics
Cloning, Molecular methods
Immunoglobulin kappa-Chains genetics
Peptide Library
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 24
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 8932399
- Full Text :
- https://doi.org/10.1093/nar/24.21.4367