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Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 1996 Nov 15; Vol. 145 (1), pp. 17-22. - Publication Year :
- 1996
-
Abstract
- An unusual filamentous bacteriophage, VSK, containing single-stranded, circular DNA as its genome was isolated from Vibrio cholerae 0139 strains P07 and B04. Unlike other single-stranded DNA phages, VSK can integrate its genome into the chromosome of the host and enter into a lysogenic state. The double-stranded replicative form (RF) of the single-stranded phage DNA was isolated. A restriction map of the VSK RF DNA was constructed using HaeII, AvaII, ClaI and XbaI. By Southern blot analysis of the chromosomal DNA of the lysogen using labeled phage DNA as probe, the attachment site (attP) on the viral genome was also identified.
- Subjects :
- Bacteriophages isolation & purification
Bacteriophages ultrastructure
Blotting, Southern
Chromosomes genetics
DNA, Bacterial analysis
DNA, Single-Stranded analysis
DNA, Single-Stranded isolation & purification
DNA, Viral analysis
Genome
Microscopy, Electron
Restriction Mapping
Vibrio cholerae genetics
Bacteriophages genetics
DNA Replication
Vibrio cholerae virology
Virus Integration
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 145
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 8931321
- Full Text :
- https://doi.org/10.1111/j.1574-6968.1996.tb08550.x