A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant.

Authors :: Mascini EM
Hazenberg MA
Verhage LA
Holm SE
Verhoef J
van Dijk H
Source :: Clinical and diagnostic laboratory immunology [Clin Diagn Lab Immunol] 1996 Nov; Vol. 3 (6), pp. 779-81.
Publication Year :: 1996
Abstract: An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.

Subjects :: Bacteriological Techniques
Culture Media analysis
Exotoxins immunology
Bacterial Proteins
Exotoxins analysis
Exotoxins isolation & purification
Membrane Proteins
Streptococcus pyogenes immunology

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