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Particulate ANP-sensitive guanylyl cyclase: induction in cultured human peripheral CD3+ mononuclear blood cells.
- Source :
-
Journal of molecular medicine (Berlin, Germany) [J Mol Med (Berl)] 1996 Oct; Vol. 74 (10), pp. 629-35. - Publication Year :
- 1996
-
Abstract
- There have been conflicting reports about the occurrence and/or activity of atrial natriuretic peptide (ANP) sensitive guanylyl cyclase in the immune system. This study reports on ANP-sensitive guanylyl cyclase mRNA expression and guanylyl cyclase activity in human peripheral blood mononuclear cells (PBMC). Reverse transcription polymerase chain reaction (RT-PCR) shows that activated human PBMC of healthy blood donors express functional active ANP-sensitive guanylyl cyclase after vitro culture, whereas freshly isolated PBMC show neither specific mRNA for particulate guanylyl cyclase nor ANP-sensitive activity of this enzyme. To define the subpopulation of PBMC expressing this enzyme, cultivated PBMC were subfractioned and analyzed by RT-PCR and in situ PCR. Only CD3+ PBMC showed mRNA for ANP-sensitive guanylyl cyclase. Induction of the guanylyl cyclase required coincubation with other cells, indicating that a factor or factors secreted from cells other than CD3+ cells induces this expression. In summary, ANP-sensitive guanylyl cyclase is an inducible enzyme in human CD3+ PBMC in contrast to other cells where it is considered to be constitutive.
- Subjects :
- Animals
Cells, Cultured
Coculture Techniques
Cyclic GMP metabolism
Guanylate Cyclase genetics
Humans
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear drug effects
Polymerase Chain Reaction
Rats
Time Factors
Atrial Natriuretic Factor pharmacology
CD3 Complex
Guanylate Cyclase metabolism
Leukocytes, Mononuclear enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0946-2716
- Volume :
- 74
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of molecular medicine (Berlin, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 8912184
- Full Text :
- https://doi.org/10.1007/s001090050067