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Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1996 Nov 01; Vol. 271 (44), pp. 27701-6. - Publication Year :
- 1996
-
Abstract
- The heterogeneous nuclear ribonucleoprotein particle (hnRNP) K protein is comprised of multiple modular domains that serve to engage a diverse group of molecular partners including DNA, RNA, the product of the proto-oncogene vav, and tyrosine and serine/threonine kinases. To identify additional K protein molecular partners and to further understand its function, we used a fragment of K protein as a bait in the yeast two-hybrid screen. The deduced primary structure of one of the positive clones revealed a novel zinc finger protein, hereby denoted as Zik1. In addition to the nine contiguous zinc fingers in the C terminus, Zik1 contains a KRAB-A domain thought to be involved in transcriptional repression. Zik1 and K protein bound in vitro and co-immunoprecipitated from cell extracts indicating that in vivo their interaction is direct. Expression of Gal4 DNA-binding domain-Zik1 fusion protein repressed a gene promoter bearing Gal4-binding elements, indicating that from cognate DNA elements Zik1 is a transcriptional repressor. The known diverse nature of K protein molecular interactions and now the identification of a K protein partner that is a transcriptional repressor lends support to the notion that K protein is a remarkably versatile molecule that may be acting as a docking platform to facilitate communication among molecules involved in signal transduction and gene expression.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
COS Cells
Cloning, Molecular
Consensus Sequence
Gene Library
Heterogeneous-Nuclear Ribonucleoprotein K
Heterogeneous-Nuclear Ribonucleoproteins
Luciferases biosynthesis
Mice
Molecular Sequence Data
Protein Biosynthesis
Rats
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Repressor Proteins biosynthesis
Saccharomyces cerevisiae
Sequence Homology, Amino Acid
Transcription, Genetic
Transfection
Nuclear Proteins
Repressor Proteins chemistry
Repressor Proteins metabolism
Ribonucleoproteins chemistry
Ribonucleoproteins metabolism
Transcription Factors
Zinc Fingers
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 271
- Issue :
- 44
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8910362
- Full Text :
- https://doi.org/10.1074/jbc.271.44.27701