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Slow-reacting and complement-requiring neutralizing antibody in swine infected with porcine reproductive and respiratory syndrome (PRRS) virus.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 1996 Aug; Vol. 58 (8), pp. 749-53. - Publication Year :
- 1996
-
Abstract
- Various conditions were evaluated and modified to enhance the sensitivity of the neutralization (NT) test for detecting antibody in swine infected with porcine reproductive and respiratory syndrome (PRRS) virus. Higher NT antibody titers were consistently obtained by the addition of 10% (v/v) complement, fresh guinea pig serum, to the virus diluent and by the incubation of serum-virus mixture at 4 degrees C for 24 hr. The appearance and persistence of antibodies detected by the modified NT test showed a similar pattern to those of antibodies detected by the indirect fluorescent antibody (IFA) assay, although the antibody titers obtained by the former method were consistently lower than those obtained by the latter method. Slow-reacting complement-requiring NT antibody was detected in sera from pig 2 weeks after infection with PRRS virus. The slow-reacting complement-requiring NT antibody in the early serum samples was sensitive to 2-mercaptoethanol (2-ME), whereas the slow-reacting complement-requiring NT antibody in the late serum samples was resistant to 2-ME. The initial phase may represent the IgM response and the later phase a change to IgG. A NT test was developed in which serum-virus mixtures were incubated at 4 degrees C for 24 hr with complement; this gave an improved sensitivity over the previous incubation at 37 degrees C for 60 min.
- Subjects :
- Animals
Cell Line
Female
Fluorescent Antibody Technique, Indirect
Guinea Pigs
Indicators and Reagents
Mercaptoethanol
Neutralization Tests methods
Neutralization Tests veterinary
Porcine Reproductive and Respiratory Syndrome blood
Porcine respiratory and reproductive syndrome virus growth & development
Porcine respiratory and reproductive syndrome virus isolation & purification
Sensitivity and Specificity
Swine
Time Factors
Antibodies, Viral blood
Complement System Proteins
Porcine Reproductive and Respiratory Syndrome immunology
Porcine respiratory and reproductive syndrome virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0916-7250
- Volume :
- 58
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 8877973
- Full Text :
- https://doi.org/10.1292/jvms.58.749