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Stimulation of fibronectin synthesis through the protein kinase C signalling pathway in normal and transformed human lung fibroblasts.
- Source :
-
Biochemistry and molecular biology international [Biochem Mol Biol Int] 1996 Aug; Vol. 39 (5), pp. 895-904. - Publication Year :
- 1996
-
Abstract
- We examined the role of the protein kinase C (PKC) signaling pathway in the stimulation of fibronectin synthesis in both normal and transformed human lung fibroblasts. Phorbol myristate acetate (PMA), a potent PKC activator, stimulated fibronectin synthesis in both normal and transformed fibroblasts in a time and dose dependent fashion. Down-regulation of PKC by prior exposure of cells to a high concentration of PMA blocked the increase in fibronectin synthesis and mRNA levels induced by PMA. Bisindolylmaleimide, a specific inhibitor of PKC, also abolished the PMA-induced fibronectin synthesis. 4 alpha-phorbol didecanoate, an inactive phorbol ester, failed to affect fibronectin synthesis. These data suggest that PMA stimulates fibronectin synthesis and gene expression through the PKC signaling pathway in both normal and transformed human lung fibroblasts.
- Subjects :
- Carcinogens pharmacology
Cell Line, Transformed
Cells, Cultured
Dose-Response Relationship, Drug
Down-Regulation
Fibroblasts drug effects
Fibronectins drug effects
Fibronectins genetics
Humans
Indoles pharmacology
Lung metabolism
Maleimides pharmacology
Phorbol Esters pharmacology
Protein Kinase C drug effects
RNA, Messenger biosynthesis
RNA, Messenger drug effects
Tetradecanoylphorbol Acetate pharmacology
Fibroblasts metabolism
Fibronectins biosynthesis
Lung cytology
Protein Kinase C metabolism
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1039-9712
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biochemistry and molecular biology international
- Publication Type :
- Academic Journal
- Accession number :
- 8866006
- Full Text :
- https://doi.org/10.1080/15216549600201042